Man-made Skin in Perspective- Principles and Applications

 Artificial Epidermis in Perspective- Concepts and Applications Dissertation

Manufactured Skin in Perspective: Ideas and Applications

Carla Abdo Brohem1, Laura Beatriz ag Silva Cardeal1, Manoela Tiago1, María T. Soengas2, Silvia Berlanga de Moraes Barros1, and Silvya Stuchi Maria-Engler1 1Department of Clinical Biochemistry & Toxicology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil

2Centro Nacional sobre Investigaciones Oncológicas (Spanish Nationwide Cancer Research Center) Madrid, Spain

Synopsis

Skin, the biggest organ from the human body, is usually organized into an elaborate layered structure consisting mainly with the outermost epidermis and the actual dermis. A subcutaneous adiposestoring hypodermis layer and several appendages including hair follicles, perspiration glands, sebaceous glands, nerve fibres, lymphatics and blood vessels are present in the skin. These multiple components of the skin ensure endurance by providing critical functions in protection, thermoregulation, excretion, absorption, metabolic functions, sensation, evaporation management and aesthetics. Study regarding how these types of biological capabilities are performed is critical in our understanding of simple skin biology, such as regulation of pigmentation and wound restore. Impairment of any of these features may lead to pathogenic alterations, which include skin cancers. Therefore , the development of genetically controlled and well-characterized skin versions can include important effects, not only to get scientists and physicians, but also for manufacturers, consumers, governing regulatory boards and animal welfare organizations. Since cells making up human skin tissue grow inside an organized three dimensional (3D) matrix continually surrounded by neighboring skin cells, standard monolayer (2D) cellular cultures do not recapitulate the physiological structures of the skin. Several types of individual skin recombinants, also called man-made skin, offering this important 3-D structure, have now recently been reconstructed in vitro. This review contemplates the use of these organotypic skin models in different applications, which includes substitutes to animal screening.

Keywords

man-made skin; skin reconstructs; skin area equivalents; rafts; organotypical nationalities; 3D versions GENERAL ARCHICTECTURE OF THE SKIN

I) Epidermal/dermal layers

The corporation of the pores and skin into epidermal and skin layers that differ in thickness, strength, and flexibility allow for a structured architecture providing you with a variety of capabilities of the skin. The outermost layer from the skin, known as the epidermis, serves as an gabardina boundary between environment and the body. Subsequently, the actual dermis is formed of solid connective muscle, which is rich in collagen and confers the characteristic versatility to the skin. Epidermis and dermis are separated by the extracellular Talk about for communication: Silvya Stuchi Maria-Engler Ph. D., Departamento de Análises Clínicas – Faculdade de Ciências Farmacêuticas, Universidade sobre São Paulo, Av. Professor Lineu Prestes, 580 Bloco 17, São Paulo – SP PAMPRE 05508-900 Brasil, Tel.: 55-11-3091-3631, Fax: 55-11-3813-2197, [email protected] br.

NIH General public Access

Author Manuscript

Pigment Cell Melanoma Res. Publisher manuscript; accessible in PMC 2012 February 1 ) Published in final edited form while:

Pigment Cell Melanoma Vaca. 2011 Feb; 24(1): 35–50. doi: 10. 1111/j. 1755-148X. 2010. 00786. x. NIH-PA Author Manuscript NIH-PA Writer Manuscript NIH-PA Author Manuscript matrix (ECM), known as the essentiel lamina (Balasubramani et ing., 2001; Horch et approach., 2005; Godin and Touitou, 2007; Ajani et al., 2007).

The epidermal part, derived from wanting ectoderm, consist of cells generated by growing keratinocytes in the stratum essentiel that move upwards when they differentiate (see Determine 1). The continuous process of proliferation, difference, and eventually, cell loss of life and shedding, allows compartmentalization into a range of strata symbolizing different levels in keratinocyte maturation (Schulz et ing., 2000; Balasubramani et 's.,...

References: THREE DIMENSIONAL human skin area equivalents. T Invest Dermatol. 2008; 128: 2498–507. [PubMed: 18528437]

a couple of

in a three-dimensional organotypic skin model. Experience Cell Ers. 2007; 313: 3005–15. [PubMed:

17512930]

anatomist. Eur J Dermatol. 08; 19: 107–13. [PubMed: 19106039]

4

will take. Med Sci (Paris). 08; 24: 607–614. [PubMed: 18601878]

8

and melanoma biology. Histol Histopathol. 2001; of sixteen: 669–74. [PubMed: 11332722]

Brohem et approach

morphogenesis following specific UVB-damages in epidermis reconstructed in vitro. Dev. Biol. 97;

183: 123–138

Differ. 1998; 5: 792–802. [PubMed: 10200539]

11

reconstructed human epidermis exposed to UVB or UV A irradiation. Photochem Photobiol. 2000;

71: 314–20

in vitro. J I am Acad Dermatol. 2008; 58: S155–9. [PubMed: 18410802]

16

extrinsic component is needed to result in the disease. Bayerischer rundfunk J Dermatol. 1997; 137: 890–7. [PubMed:

9470904]

Eur J Cellular Biol. 2007; 86: 731–746. [PubMed: 17292509]

18

spontaneously transformed keratinocyte cell range (HaCaT). J Invest Dermatol. 1999; 112: 489–98.

types: morphological and immunohistochemical analysis. Acta Derm Venereol. 2k; 80: 82–

8

20. Botham PA. The validation of in vitro options for skin irritation. Toxicol Lett. 2004; 149: 387–90.

skin area substitutes. Are J Surg. 2002; 183: 445–56. [PubMed: 11975935]

twenty two

Am L Surg. 2008; 195: 144–8. [PubMed: 18070722]

23

effect on individual epidermal skin discoloration. Pigment Cellular Res. 06\; 19: 434–42. [PubMed:

16965272]

interactions during tumourigenesis. Fibrogenesis Tissue Restore. 2008; one particular: 8. [PubMed: 19077226]

twenty six

biodegradable polyglycolic acid or polyglactin fine mesh. Biomaterials. 1991; 12: 243–248. [PubMed:

1652296]

micronucleus debut ? initiation ? inauguration ? introduction in a 3D human skin area model (EpiDerm). Mutat Ers. 2006; 607: 192–204.

overview. Br L Dermatol. 2009; 161: 19–24. [PubMed: 19466960]

31

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